5-Amino Mixing & Dosage Protocols

5-Amino-1MQ (50 mg) — Research Dosage Guidance

Metabolic Acceleration • Fat Loss • Cellular Energy Optimization

Reconstitution (Mixing)

  • Add 2 ml Bacteriostatic Water to the vial
  • Roll gently between palms (do not shake)
  • Store reconstituted vial in the refrigerator (2–8 °C)

Concentration Calculation

50 mg ÷ 2 ml = 25 mg/ml

Every 10 units (0.1 ml) = 2.5 mg

Each 1 mg = 4 units on a U-100 insulin syringe

Core Dosage Guidelines

For metabolic acceleration, fat loss, and cellular energy research

Standard research protocol: 5–10 mg daily

Frequency: Once daily, 5 days per week (e.g. Monday–Friday)

Administration: Subcutaneous (abdomen, thigh, or glute)

Injection sites: Rotate regularly

Syringe Measurement Guide
Desired Dose    Volume (ml)    Insulin Units
2.5 mg    0.10 ml    10 units
5 mg    0.20 ml    20 units
7.5 mg    0.30 ml    30 units
10 mg    0.40 ml    40 units


Targeted Research Protocols (Different Situations)
1.⁠ ⁠Fat-Loss & Body-Composition Research

  • (Lipolysis, adipose reduction models)
  • Dose: 5–10 mg
  • Frequency: Once daily, 5× weekly
  • Duration: 6–8 weeks
  • Notes: Core use-case for 5-Amino-1MQ

2.⁠ ⁠Metabolic Health & Insulin Sensitivity

  • (Glucose handling, metabolic flexibility)
  • Dose: 5 mg
  • Frequency: Once daily, 5× weekly
  • Duration: 4–6 weeks
  • Notes: Lower dose often sufficient

3.⁠ ⁠Energy & Cellular NAD⁺ Support

  • (NNMT inhibition, mitochondrial efficiency)
  • Dose: 5–7.5 mg
  • Frequency: Once daily
  • Duration: 6–8 weeks
  • Notes: Synergistic with NAD⁺

4.⁠ ⁠Lean-Mass Preservation & Muscle Definition

  • (Recomposition, calorie-restricted models)
  • Dose: 7.5–10 mg
  • Frequency: Once daily
  • Duration: 6–8 weeks
  • Notes: Supports muscle definition while reducing fat

5.⁠ ⁠Longevity & Mitochondrial Optimization

  • (Cellular resilience, metabolic aging pathways)
  • Dose: 5 mg
  • Frequency: 3–5× weekly
  • Duration: 8 weeks
  • Notes: Often alternated with MOTS-C cycles

6.⁠ ⁠Maintenance / Long-Term Metabolic Support

  • Dose: 2.5–5 mg
  • Frequency: 3× weekly
  • Duration: Ongoing after full cycle
  • Notes: Maintains metabolic signaling with minimal exposure

Cycle Duration

  • Typical cycle: 4–8 weeks
  • Break: 2 weeks
  • Repeat cycles: May be repeated or alternated with NAD⁺ or MOTS-C for enhanced mitochondrial and metabolic support

Key Research Benefits

  • Inhibits NNMT, supporting higher cellular NAD⁺ levels
  • Promotes fat reduction and improved insulin sensitivity
  • Enhances cellular energy metabolism
  • Supports muscle definition and metabolic balance
  • Complements longevity-focused research stacks

Important Notes

Always use sterile needles and alcohol swabs

Do not reuse or share needles

Store reconstituted vial at 2–8 °C

For research use only