GHK-CU Mixing and dosage protocols

GHK-Cu (Copper Peptide) 100 mg — Research Dosage Guidance

Skin Rejuvenation • Wound Healing • Hair Growth • Anti-Aging Research

Reconstitution (Mixing)

  • Add 3 ml Bacteriostatic Water to the vial
  • Roll gently between palms (do not shake)
  • Store reconstituted vial in the refrigerator (2–8 °C)

Concentration Calculation

100 mg ÷ 3 ml ≈ 33.3 mg/ml

Every 10 units (0.1 ml) ≈ 3.3 mg

Core Dosage Guidelines

For skin regeneration, tissue repair, hair stimulation, and longevity-focused research

Injectable skin therapy: 250 mcg – 3 mg per day

Topical / microneedling research: 0.5 – 3 mg per session

Do not exceed: 3 mg per day

⚠️ Higher daily exposure may increase angiogenesis and copper accumulation, particularly in long-term protocols or in subjects with impaired copper clearance.

Syringe Measurement Guide
Dose    Volume (ml)    Insulin Units
250 mcg    ≈ 0.007 ml    < 1 unit
500 mcg    ≈ 0.015 ml    1.5 units
1 mg    ≈ 0.03 ml    3 units
2 mg    ≈ 0.06 ml    6 units
3 mg (maximum)    ≈ 0.09 ml    9 units

Accuracy note: For doses under 0.1 ml, use 30–50 unit insulin syringes.

Targeted Research Protocols (Different Situations)
1.⁠ ⁠Skin Rejuvenation & Anti-Aging Research

  • (Dermal thickness, elasticity, collagen renewal)
  • Dose: 250–500 mcg
  • Frequency: Once daily or every other day
  • Route: Subcutaneous or intradermal
  • Duration: 6–10 weeks
  • Notes: Low-dose protocols often sufficient for cosmetic endpoints

2.⁠ ⁠Wound Healing & Tissue Repair

  • (Post-procedure healing, scar modulation)
  • Dose: 1–2 mg
  • Frequency: Once daily
  • Route: Local subcutaneous or intradermal near target area
  • Duration: 4–8 weeks
  • Notes: Supports angiogenesis and fibroblast activity

3.⁠ ⁠Hair Growth & Scalp Regeneration

  • (Follicular support, vascular supply, hair density)
  • Dose: 500 mcg – 1.5 mg
  • Frequency: 3–5× weekly
  • Route: Intradermal scalp administration 
  • Duration: 8–12 weeks
  • Notes: Avoid excessive daily dosing to limit vascular overgrowth

4.⁠ ⁠Intensive Skin Repair / Post-Laser or Microneedling Research

  • (Barrier recovery, collagen induction)
  • Dose: 1–3 mg
  • Frequency: On treatment days only
  • Route: Intradermal or topical application to treated area
  • Duration: As per procedural protocol
  • Notes: Do not exceed 3 mg/day

5.⁠ ⁠Longevity & Cellular Repair Research

  • (Fibroblast signaling, tissue resilience)
  • Dose: 250–500 mcg
  • Frequency: 3–5× weekly
  • Duration: 8–10 weeks
  • Notes: Suitable for long-term, low-dose protocols

6.⁠ ⁠Maintenance / Long-Term Skin Support

  • Dose: 250–500 mcg
  • Frequency: Every other day or 2–3× weekly
  • Duration: Ongoing after full cycle
  • Notes: Minimizes copper load while maintaining benefits

Copper-Related Safety Considerations

Excessive or prolonged exposure above 3 mg/day, especially when combined with high-dose copper supplementation, may increase risk of copper accumulation.

Possible signs of copper overload include:

  • Fatigue or nausea
  • Metallic taste
  • Elevated liver enzymes (AST/ALT)
  • Neurological irritability or confusion
  • Use caution in research models involving:
  • Impaired liver function
  • Copper metabolism disorders (e.g. Wilson-type models)

Cycle Duration

  • Typical cycle: 4–10 weeks
  • Maintenance: Lower doses (250–500 mcg) daily or every other day
  • Repeat cycles: May be repeated with appropriate washout periods

Key Research Benefits

  • Stimulates collagen and extracellular matrix renewal
  • Enhances wound healing and tissue regeneration
  • Supports hair follicle vascularization and growth
  • Improves skin texture, elasticity, and repair capacity
  • Activates regenerative and longevity-associated pathways

Important Notes

Always use sterile needles and alcohol swabs

Do not reuse or share needles

Store reconstituted vial at 2–8 °C

For research use only