Glutathione Mixing & Dosage Protocols

Glutathione (GSH) — Research Dosage Guidance
Reconstitution (Mixing)

  • Add 3.0 ml Bacteriostatic Water to the vial
  • Final concentration: 500 mg per ml
  • Roll gently to dissolve (do not shake)
  • Store in the refrigerator (2–8 °C)

Concentration Calculation

1,500 mg ÷ 3 ml = 500 mg/ml

Every 1 insulin unit (0.01 ml) = 5 mg Glutathione

Core Dosage Guidelines (Subcutaneous)

  • Target weekly total: 1,500 mg
  • Injection frequency: 3× weekly

Standard Dosing Schedule

Weeks 1–2 (Initiation)

300 mg per injection

0.60 ml (60 units)

900 mg per week

Weeks 3–12 (Full Protocol)

500 mg per injection

1.0 ml (100 units)

1,500 mg per week

Optional Weeks 12–16

500 mg per injection

1.0 ml (100 units)

1,500 mg per week

Example injection days: Monday – Wednesday – Friday

Dose Conversion Table
Dose    mL    Units
300 mg    0.60 ml    60 units
500 mg    1.0 ml    100 units


Targeted Research Protocols (Different Situations)
1.⁠ ⁠Antioxidant & General Wellness Research

  • (Oxidative stress reduction, cellular protection models)
  • Dose: 250–300 mg
  • Frequency: 2–3× weekly
  • Weekly total: 500–900 mg
  • Duration: 6–10 weeks
  • Notes: Suitable for maintenance or first-time research models

2.⁠ ⁠Immune Support & Inflammation Research

  • (Immune modulation, recovery support)
  • Dose: 300–500 mg
  • Frequency: 3× weekly
  • Weekly total: 900–1,500 mg
  • Duration: 8–12 weeks
  • Notes: Aligns with standard protocol

3.⁠ ⁠Liver Support & Detoxification Research

  • (Hepatic stress, toxin exposure models)
  • Dose: 500 mg
  • Frequency: 3× weekly
  • Weekly total: 1,500 mg
  • Duration: 8–16 weeks
  • Notes: Ensure adequate hydration in research models

4.⁠ ⁠Intensive Oxidative Stress / Recovery Models

  • (Post-illness, high metabolic stress, advanced research)
  • Dose: 500 mg
  • Frequency: 3× weekly
  • Weekly total: 1,500 mg
  • Duration: 12–16 weeks
  • Notes: Advanced protocol only

5.⁠ ⁠Skin, Pigmentation & Cellular Aging Research

  • (Melanin modulation, skin clarity, anti-aging pathways)
  • Dose: 300–500 mg
  • Frequency: 2–3× weekly
  • Weekly total: 600–1,500 mg
  • Duration: 8–12 weeks
  • Notes: Gradual onset protocols preferred

6.⁠ ⁠Maintenance / Long-Term Support Research

  • Dose: 250–300 mg
  • Frequency: 1–2× weekly
  • Weekly total: 250–600 mg
  • Duration: Ongoing after a full cycle

Vial Usage

  • Each vial contains 1,500 mg total
  • 3 × 500 mg doses per vial
  • 1 vial = 1 full week during full protocol

Reconstitution Steps

  • Draw 3.0 ml bacteriostatic water
  • Inject slowly down the vial wall to prevent foaming
  • Swirl gently until fully dissolved
  • Refrigerate at 2–8 °C immediately
  • Do not freeze once reconstituted
  • Solution should be clear to pale yellow

Cycle Duration

Standard protocol: 8–12 weeks

Optional extension: Up to 16 weeks

Research Use Disclaimer

For research and educational purposes only.
Not intended to diagnose, treat, or cure any condition.