NAD+ Mixing & Dosage Protocols

NAD⁺ (Nicotinamide Adenine Dinucleotide) — Research Dosage Guidance

Cellular Energy • Mitochondrial Support • Longevity Research

Reconstitution (Mixing)

  • Add 3.0 ml Bacteriostatic Water to the vial
  • Roll gently between palms (do not shake)
  • Store reconstituted vial in the refrigerator (2–8 °C)

Concentration Calculation

500 mg ÷ 3 ml = ~166.7 mg per ml

Every 10 units (0.1 ml) ≈ 16.7 mg NAD⁺

Each 1 unit (0.01 ml) ≈ 1.67 mg NAD⁺

Core Dosage Guidelines

For mitochondrial support, cellular rejuvenation, and longevity-pathway research

General research dose: 25–100 mg per injection

Frequency: 3–5× weekly

Administration routes:

Subcutaneous (abdomen, thigh, glute)

Intramuscular or slow IV (controlled research environments only)

Syringe Measurement Guide
Desired Dose    Volume (ml)    Insulin Units
10 mg    0.06 ml    6 units
25 mg    0.15 ml    15 units
50 mg    0.30 ml    30 units
75 mg    0.45 ml    45 units
100 mg    0.60 ml    60 units

Reference:

1 unit ≈ 1.67 mg NAD⁺

10 units ≈ 16.7 mg NAD⁺

Targeted Research Protocols
1.⁠ ⁠Energy & Fatigue Recovery Research

  • Dose: 25–50 mg
  • Frequency: 2–3× weekly
  • Duration: 4–6 weeks

2.⁠ ⁠Cognitive & Neuro-Energy Research

  • Dose: 25–75 mg
  • Frequency: 3× weekly
  • Duration: 4–8 weeks

3.⁠ ⁠Longevity & Anti-Aging Pathway Research

  • Dose: 50–100 mg
  • Frequency: 3–5× weekly
  • Duration: 6–8 weeks

4.⁠ ⁠Metabolic & Weight-Optimization Research

  • Dose: 50 mg
  • Frequency: 3–5× weekly
  • Notes: Synergistic with 5-Amino-1MQ, MOTS-C, GLOW or KLOW Blend

5.⁠ ⁠Maintenance / Long-Term Support

  • Dose: 25–50 mg
  • Frequency: 2–3× weekly
  • Duration: Ongoing after full cycle

Cycle Duration

  • Typical cycle: 4–8 weeks
  • Maintenance: 25–50 mg, 2–3× weekly
  • Repeat cycles: As required for research goals

Important Notes

Always use sterile needles and alcohol swabs

Do not reuse or share needles

Store reconstituted vial at 2–8 °C

For research use only