PT-141 Mixing & Dosage Protocols

PT-141 (10 mg) — Research Dosage Guidance


Reconstitution (Mixing)

  • Add 2 ml Bacteriostatic Water to the vial
  • Roll gently between palms (do not shake)
  • Store reconstituted vial in the refrigerator (2–8 °C)

Concentration Calculation

10 mg ÷ 2 ml = 5 mg/ml

Every 10 insulin units (0.1 ml) = 0.5 mg (500 mcg)

Core Dosage Guidelines

For libido, mood, and energy-pathway research

Standard research dose: 500 mcg – 2 mg per injection

Typical starting dose: 500 mcg (0.1 ml / 10 units)

Maximum single dose: 2 mg (0.4 ml / 40 units)

Administration: Subcutaneous (abdomen or thigh)

Timing: Inject 45–60 minutes before the desired effect window

Syringe Measurement Guide
Dose    Volume (ml)    Insulin Units
250 mcg    0.05 ml    5 units
500 mcg    0.10 ml    10 units
1 mg    0.20 ml    20 units
2 mg    0.40 ml    40 units

Reference: Every 10 units (0.1 ml) = 500 mcg PT-141

Targeted Research Protocols (Different Situations)
1.⁠ ⁠Libido Initiation / First-Exposure Research

  • (Sensitivity assessment, response profiling)
  • Dose: 500 mcg
  • Frequency: As needed
  • Timing: 45–60 minutes prior
  • Notes: Preferred starting protocol to assess tolerance

2.⁠ ⁠Standard Libido & Sexual-Response Research

(Male & female arousal, desire, and response models)

  • Dose: 1 mg
  • Frequency: As needed
  • Timing: 45–60 minutes prior
  • Notes: Most commonly used research dose

3.⁠ ⁠Reduced-Response / Advanced Libido Research

  • (Lower responder models, refractory libido)
  • Dose: 1.5 – 2 mg
  • Frequency: As needed
  • Timing: 45–60 minutes prior
  • Notes: Advanced protocol; monitor for nausea or flushing

4.⁠ ⁠Mood & Motivation Pathway Research

  • (Melanocortin-mediated mood and reward signaling)
  • Dose: 500 mcg – 1 mg
  • Frequency: 1–2× weekly
  • Timing: Morning or early evening
  • Notes: Lower doses often sufficient for mood effects

5.⁠ ⁠Energy & Drive Modulation Research

  • (Central motivation, vitality signaling)
  • Dose: 500 mcg – 1 mg
  • Frequency: 1–2× weekly
  • Timing: Morning preferred
  • Notes: Non-stimulant mechanism

Cycle & Frequency Guidelines

Use as needed

Do not exceed: Once per 24 hours

Typical research cycle: 2–3× weekly

Rest period: Allow ≥24 hours between doses

Key Research Benefits

  • Activates melanocortin receptors MC3R & MC4R
  • Enhances libido and sexual response in male and female models
  • Supports mood and motivational signaling
  • Non-hormonal and independent of nitric-oxide pathways
  • Distinct mechanism compared to PDE-5 inhibitors

Important Notes

Always use sterile needles and alcohol swabs

Do not reuse or share needles

Store reconstituted peptide at 2–8 °C

For research use only

Possible dose-dependent effects:

Temporary flushing

Mild nausea

Headache in sensitive research models