Semaglutide Mixing & Dosage Protocols

(GLP-1 Receptor Agonist for Weight Management & Metabolic Research)

Reconstitution (Mixing)

  • Add 2.5 ml Bacteriostatic Water to the vial.
  • Roll gently between your palms (do not shake).
  • Store in the refrigerator after mixing.

 Concentration Calculation

10 mg ÷ 2.5 ml = 4 mg per ml

Every 10 units (0.1 ml) = 0.4 mg (400 mcg)

 Dosage Guidelines

For appetite regulation, weight management, and glucose balance research:

  • Starting dose: 0.25 mg (≈6 units) once per week
  • Increase gradually:
    • Week 3–4 → 0.5 mg (≈13 units) weekly
    • Week 5–6 → 1.0 mg (≈25 units) weekly
    • Week 7+ → 2 mg or as tolerated
  • Administration: Subcutaneous injection (abdomen, thigh, or upper arm)
  • Frequency: Once weekly on the same day each week

Biopeptics recommendation:

Always start by assessing tolerance with a micro-dose of 0.25 mg, and stay on the lowest effective dose for as long as possible. Some individuals may not need to increase their dosage at all - only adjust the dose if truly necessary, and do so in very small increments.

Key Benefits:

  • Promotes satiety and reduced appetite
  • Supports blood-sugar balance and fat-loss research
  • May improve cardiovascular and metabolic markers
  • Encourages stable energy levels and weight control

Syringe Measurement Guide

Dose

Volume (ml)

Insulin Units (U-100 Syringe)

0.25 mg

0.06 ml

6 units

0.5 mg

0.13 ml

13 units

1 mg

0.25 ml

25 units

2 mg

0.50 ml

50 units

Each 10 units (0.1 ml) = 0.4 mg Semaglutide

Cycle Duration

  • Minimum effective duration: 8–12 weeks
  • Ongoing protocol: Continue weekly maintenance once goal is achieved
  • Adjust or pause only under professional research guidance.

 Important Notes

  • Always use sterile needles and alcohol swabs.
  • Do not reuse or share needles.
  • Store mixed vial in the refrigerator (2–8 °C).
  • For research use only.
  • Common transient reactions: mild nausea, reduced appetite, or injection-site redness.