NAD⁺ - 500 mg (β-Nicotinamide Adenine Dinucleotide)

1. Cellular Energy & Mitochondrial Function

NAD⁺ is the primary electron carrier in redox reactions essential for ATP production. It participates in key cellular cycles:

• Glycolysis (glucose to pyruvate conversion)
• Krebs / TCA Cycle (energy release within mitochondria)
• Oxidative phosphorylation (ATP synthesis via electron-transport chain)

By maintaining mitochondrial efficiency, NAD⁺ ensures optimal cellular energy output, resilience, and survival under metabolic stress.

2. DNA Repair & Sirtuin Activation

NAD⁺ acts as a substrate for sirtuins (SIRT1–SIRT7) -a family of longevity-associated enzymes that regulate:

• Gene expression linked to aging and metabolism
• DNA-damage repair through PARP (poly ADP-ribose polymerase) activation
• Telomere maintenance and genome stability
• Cellular stress response and inflammation control

Sirtuin activation via NAD⁺ enhances cellular self-repair and supports youthful metabolic function in research models.

3. Neuroprotection & Cognitive Function

NAD⁺ supports neuronal energy balance and neurotransmitter synthesis. It promotes axon regeneration, stabilizes synaptic signaling, and protects against oxidative and excitotoxic stress. Research shows elevated NAD⁺ levels correlate with improved cognitive performance, focus, and neuroplasticity.

4. Anti-Inflammatory & Metabolic Regulation

By influencing NF-κB and AMPK pathways, NAD⁺ helps:

• Reduce chronic inflammation at the cellular level
• Improve glucose utilization and lipid metabolism
• Support circadian rhythm and metabolic homeostasis

This makes NAD⁺ an integral molecule in aging, metabolic-syndrome, and mitochondrial-function studies.

• Mitochondrial energy-metabolism research
• DNA-repair and sirtuin-activation studies
• Neurodegenerative-disease and cognitive-function models
• Anti-inflammatory and longevity research
• Metabolic and fatigue-recovery trials

NAD⁺ (β-Nicotinamide Adenine Dinucleotide) -500 mg Lyophilized Powder

NAD⁺ (Nicotinamide Adenine Dinucleotide) — Research Dosage Guidance

Cellular Energy • Mitochondrial Support • Longevity Research

Reconstitution (Mixing)

• Add 3.0 ml Bacteriostatic Water to the vial
• Roll gently between palms (do not shake)
• Store reconstituted vial in the refrigerator (2–8 °C)

Concentration Calculation

500 mg ÷ 3 ml = ~166.7 mg per ml

Every 10 units (0.1 ml) ≈ 16.7 mg NAD⁺

Each 1 unit (0.01 ml) ≈ 1.67 mg NAD⁺

Core Dosage Guidelines

For mitochondrial support, cellular rejuvenation, and longevity-pathway research

General research dose: 25–100 mg per injection

Frequency: 3–5× weekly

Administration routes:

Subcutaneous (abdomen, thigh, glute)

Intramuscular or slow IV (controlled research environments only)

Syringe Measurement Guide
Desired Dose    Volume (ml)    Insulin Units
10 mg    0.06 ml    6 units
25 mg    0.15 ml    15 units
50 mg    0.30 ml    30 units
75 mg    0.45 ml    45 units
100 mg    0.60 ml    60 units

Reference:

1 unit ≈ 1.67 mg NAD⁺

10 units ≈ 16.7 mg NAD⁺

Targeted Research Protocols
1.⁠ ⁠Energy & Fatigue Recovery Research

• Dose: 25–50 mg
• Frequency: 2–3× weekly
• Duration: 4–6 weeks

2.⁠ ⁠Cognitive & Neuro-Energy Research

• Dose: 25–75 mg
• Frequency: 3× weekly
• Duration: 4–8 weeks

3.⁠ ⁠Longevity & Anti-Aging Pathway Research

• Dose: 50–100 mg
• Frequency: 3–5× weekly
• Duration: 6–8 weeks

4.⁠ ⁠Metabolic & Weight-Optimization Research

• Dose: 50 mg
• Frequency: 3–5× weekly
• Notes: Synergistic with 5-Amino-1MQ, MOTS-C, GLOW or KLOW Blend

5.⁠ ⁠Maintenance / Long-Term Support

• Dose: 25–50 mg
• Frequency: 2–3× weekly
• Duration: Ongoing after full cycle

Cycle Duration

• Typical cycle: 4–8 weeks
• Maintenance: 25–50 mg, 2–3× weekly
• Repeat cycles: As required for research goals

Important Notes

Always use sterile needles and alcohol swabs

Do not reuse or share needles

Store reconstituted vial at 2–8 °C

For research use only

NAD⁺ is a central metabolic cofactor involved in mitochondrial function, DNA repair, and cellular energy regulation. Use caution or avoid in the following research contexts:

Pregnancy or lactation models (no safety data available)

Severe hypotension-prone models (may further lower blood pressure; monitor closely)

Advanced liver disease or cirrhosis models (impaired nicotinamide metabolism)

Gout or hyperuricemia-sensitive models (high NAD⁺ turnover may elevate uric acid)

Diabetes or hypoglycemia-prone models (monitor glucose due to improved insulin sensitivity)

Active cancer or tumor-bearing models (NAD⁺ supports DNA repair and mitochondrial pathways that may confound proliferation studies)

NAD⁺ is a naturally occurring molecule with a high safety margin in research when properly diluted and administered slowly. Most reported side effects -flushing, dizziness, mild nausea, or fatigue -are transient and dose-related. Severe or prolonged discomfort usually indicates too rapid infusion, inadequate dilution, or excessive concentration.