TB-500 - 10mg (Thymosin Beta-4)

1. Actin Regulation and Cell Motility

TB-500’s core mechanism centers on its ability to bind and regulate actin, a fundamental protein that forms the cell’s structural framework. By controlling actin polymerization, TB-500:

• Stimulates cell migration and differentiation at injury sites.
• Facilitates tissue remodeling and wound closure.
• Enhances communication between repair cells, including fibroblasts and keratinocytes.

This enables cells to move more efficiently to damaged regions, jump-starting the body’s innate recovery processes.

2. Angiogenesis and Nutrient Delivery

TB-500 upregulates vascular endothelial growth factor (VEGF) and related pathways that drive angiogenesis -the creation of new blood vessels. This effect supports:

• Improved oxygen and nutrient transport to injured tissue.
• Enhanced capillary formation for long-term vascular integrity.
• Greater metabolic activity in regenerating areas.

In research contexts, this results in faster tissue recovery and reduced ischemic damage.

3. Inflammation Modulation

TB-500 helps regulate inflammatory signaling by:

• Suppressing excess cytokine production (e.g., IL-1, TNF-α).
• Supporting anti-fibrotic and antioxidant pathways.
• Reducing tissue stiffness and oxidative stress within the microenvironment.

This anti-inflammatory activity promotes smoother healing and minimizes scar-tissue formation in chronic-injury models.

4. Muscle and Connective Tissue Support

Through its action on myocytes, fibroblasts, and satellite cells, TB-500:

• Encourages muscle-fiber repair and regrowth.
• Improves tendon and ligament flexibility.
• Enhances joint mobility and resilience after strain or surgery.

The result is improved recovery potential for both musculoskeletal and soft-tissue systems.

• Muscular and tendon-injury recovery models
• Inflammation-modulation studies
• Angiogenesis and vascular-growth research
• Post-surgical tissue-repair protocols
• Sports-recovery and flexibility investigations
• Fibrosis-reduction and scar-healing analysis

TB-500 (Thymosin Beta-4) -10 mg lyophilized powder

TB-500 (10 mg) — Research Dosage Guidance

Tissue Repair • Recovery • Performance Support

• Reconstitution (Mixing)
• Add 2 ml Bacteriostatic Water to the vial
• Roll gently between palms (do not shake)
• Store reconstituted vial in the refrigerator (2–8 °C)

Concentration Calculation

10 mg ÷ 2 ml = 5 mg/ml

Every 10 insulin units (0.1 ml) = 0.5 mg (500 mcg)

Core Dosage Guidelines

For injury repair, recovery, and performance-support research

Acute injury / post-surgical repair: 1–2 mg, 3× weekly

Maintenance / general wellbeing: 250–500 mcg, 2–3× weekly

Administration: Subcutaneous or intramuscular; if possible, administer near the affected area

Injection sites: Rotate regularly

Dose Conversion Table

Dose    Volume    Insulin Units

250 mcg    0.05 ml    5 units
500 mcg    0.10 ml    10 units
1 mg    0.20 ml    20 units
2 mg    0.40 ml    40 units

Reference: Every 10 units (0.1 ml) = 500 mcg TB-500

Targeted Research Protocols (Different Situations)
1.⁠ ⁠Acute Injury & Surgical Recovery

• (Tendon/ligament tears, post-procedure healing)
• Dose: 1–2 mg
• Frequency: 3× weekly
• Duration: 4–6 weeks
• Notes: IM or SC near affected area when appropriate

2.⁠ ⁠Chronic Pain, Overuse & Inflammation

• (Tendinopathy, repetitive strain, joint stress)
• Dose: 500 mcg – 1 mg
• Frequency: 2–3× weekly
• Duration: 6–8 weeks
• Notes: Consistent dosing preferred over higher spikes

3.⁠ ⁠Muscle Recovery & Athletic Performance

• (Training load, micro-trauma recovery)
• Dose: 1 mg
• Frequency: 2× weekly
• Timing: Post-training or rest days
• Duration: 6–8 weeks

4.⁠ ⁠Soft-Tissue & Mobility Support

• (Fascial health, flexibility, range of motion)
• Dose: 500 mcg
• Frequency: 2–3× weekly
• Duration: 6 weeks
• Notes: Often paired with physiotherapy models

5.⁠ ⁠Maintenance / Preventative Research

• Dose: 250–500 mcg
• Frequency: 2× weekly
• Duration: Ongoing after recovery
• Notes: Maintains tissue resilience with minimal exposure

Cycle Duration

• Active recovery cycle: 4–8 weeks (based on severity)
• Maintenance: 250–500 mcg, 2× weekly thereafter
• Repeat cycles: May be repeated as required for research objectives

Key Research Benefits

• Accelerates tissue repair and cellular migration
• Supports tendon, ligament, and muscle recovery
• Enhances flexibility and mobility
• Assists recovery from strain and repetitive stress
• Complements regenerative research stacks (e.g., BPC-157, GHK-Cu)

Important Notes

Always use sterile needles and alcohol swabs

Do not reuse or share needles

Store reconstituted solution at 2–8 °C

For research use only

TB-500
Who Should Use with Caution / Avoid in Research Contexts
Active or recent cancer models -TB-500 promotes angiogenesis and cell proliferation.
Severe edema, heart failure, or renal disease -risk of fluid retention.
Pregnancy / breastfeeding -no established safety data.
Uncontrolled hypertension -monitor for vascular reactivity.
Diabetic retinopathy or proliferative eye disease -angiogenic pathways could worsen symptoms.

TB-500 displays low toxicity and is well tolerated in most research contexts. Transient fatigue, injection-site warmth, or mild edema are the most common observations. Severe or prolonged symptoms often indicate injection-technique issues or excessive local angiogenesis and resolve after dilution or discontinuation.